Intracellular Protein Editing
We recently established a method for editing the primary amino acid sequence of proteins in live mammalian cells. This approach enables the site-specific editing of noncanonical amino acids and useful labels into target proteins, including endogenous proteins. Ultimately, this enables the labeling and manipulation of proteins in a nondisruptive and temporally resolved fashion. This protein editing method represents a useful and widely applicable addition to the repertoire of technologies for studying proteins in mammalian cells, offering the potential to edit, manipulate, and observe nearly limitless target proteins across diverse applications.
